Gareth Bale is out of the Wales squad to ‘ friendly match against Mexico in New York at the end of the month.
The Spurs midfielder, 22, along with four other members of the original squad “have minor injury concerns and it has been agreed that they will not travel,” said a statement from the FAW.
Midfielders Joe Ledley and David Vaughan, and defenders Neal Eardley and Darcy Blake are the other wthdrawls from Chris Coleman’s first match in charge.
Goalkeeper, Lewis Price, has also pulled out of the sqaud.
Nottingham Forest defender, Joel Lynch, was added to the squad on Thursday after the FAW received confirmation of his eligibility through his Barry born father.
And defenders Adam Henley and Ashley Richards have also be called into the main squad for the first time, along with Crystal Palace striker, Jermaine Easter and Tranmere Rovers goalkeeper, Owain Fôn Williams.
Henley, 17, gets his chance after a good end to his season with Blackburn Rovers, which included an excellent performance in his side’s 3-2 victory over Manchester United at Old Trafford.
He made his first appearance for the Wales under-21 side in the 4-0 win over Andorra at the Racecourse in February, but he is still also eligible for the USA as he was born in Knoxville, Tennessee.
Richards, 22, played nine times for Swansea City last season in the Premiership and the FA Cup as well as leading the Under 21 side in their Euro 2013 qualifiers.
The game will be held at the MetLife Stadium, New York, home of American Football teams, the New York Jets and New York Giants and the venue for the 2014 Super Bowl.
This will be the fourth time Wales have played a match in a third country having faced Brazil at White Hart Lane, London in 2005, Triniadad & Tobago in Graz, Austria in 2006 and Poland in Vila Real, Portugal in 2009.
Wales squad to face Mexico on 27 May:
Jason Brown (Aberdeen), Owain Fôn Williams (Tranmere Rovers), Rhys Taylor (Chelsea); Chris Gunter (Nottingham Forest), Neil Taylor (Swansea City), Sam Ricketts (Bolton Wanderers), Ashley Williams (Swansea City), Adam Matthews (Celtic), Joel Lynch (Nottingham Forest), Adam Henley (Blackburn Rovers); Joe Allen (Swansea City), Aaron Ramsey (Arsenal), Andrew Crofts (Norwich City), David Vaughan (Sunderland), Joe Ledley (Celtic), Andy King (Leicester City), David Edwards (Wolverhampton Wanderers); Craig Bellamy (Liverpool), Hal Robson-Kanu (Reading), Steve Morison (Norwich City), Simon Church (Reading), Sam Vokes (Wolverhampton Wanderers), Jermaine Easter (Crystal Palace).
The game will be LIVE on S4C Sunday 27 May at 8:00pm